bone scintigraphy

principles

·      uses technietium-99m attached to methylene diphosphonate (Tc-99m MDP)

·      relies on blood flow to and within bone

·      then relies on surface apposition of Tc-99 MDP on to hydroxyapatite crystal matrix

·      increased bone tracer activity due to increased hydroxyapatite crystal turnover

·      can be due to

·      increased blood flow

·      growth

·      remodelling

·      repair in response to inflammation / trauma / neoplasia

mechanism of uptake

·      three phases to uptake

First phase

·      isotope diffuses passively through bone capillaries

·      dependent on concentration gradient between intra and extravascular compartments

·      increased local blood flow results in increased local flux of radionculide at affected site

Second phase

·      radionuclide concentrated in bone

·      mechanism not fully inderstood

·      some uptake in normal bone

·      radionuclide avidly concentrated in new woven bone

·      associated with increased osteoblastic activity

·      taken up against a concentration gradient

Bone scan

·      Tc-99 MDP is water soluble complex

·      one half is excreted in urine

·      remainder predominantly concentrated in skeleton

Phases
First phase

·      syn. dynamic or arterial images

·      series of rapid sequential regional images in 1st few seconds after injection of dye

·      reflect initial blood flow

·      useful when infection suspected

Second phase

·      syn. blood pool or venous images

·      early equilibrium image at 5 min

·      reflects blood and extracellular concentrations

·      indicates vascularity of region

Third phase

·      syn. bone images

·      at 2.5-3 hrs

Resolution

·      increased by using

·      multiple projections

·      collimator

·      affected part as close as possible to collimator

·      tomography

Collimator

·      pin-hole collimator views provide greater definition of small areas

·      used for

·      hands or wrists

·      areas near growth plates in children

·      localised area (eg. osteoid osteoma)

SPECT

·      single photon emmission computed tomography

·      gamma camera rotated around patient

·      provides localisation of abnormalities in axial, coronal or sagittal planes

·      better contrast resolution

Uses

·      vertebral column

·      pars defects

·      apophyseal disease

·      solitary metastasis

·      hip joints

·      AVN

·      knees

·      ruptured ACL

·      OCD

Exposure

·      exposure is 6 mSv

·      kidney and bladder receive higher dose of radiation

·      dose to critical organs (marrow, gonads) similar to lumbar spine x-rays or CT of lumbar spine or pelvis

Specialised scanning
Gallium scan

·      gallium-67 citrate is isotope that binds to white cells

·      injected into circulation

·      6% bound

·      remainder excreted into bowel

·      leads to increased concentration in infection

·      abscess

·      osteomyelitis

·      also accumulates in

·      inflammation

·      tumours

·      fractures

·      normal bone marrow

·      interpretation requires comparison with technetium scan

·      any inflammatory condition will concentrate gallium

·      there is a low level of normal activity of bone

·      thus infection likely when gallium intensity greater than that on technetium scan

·      2-3 day delay between injection and imaging required

White cell labelled scan

·      patient's white cells separated and labelled with radioactive agent

·      with Tc-99m in Australia

·      Indium-111 gives better scans but not available in Australia for this

·      accumulates in

·      abscess

·      osteomyeleitis

·      inflammation

·      tumours

·      fractures

·      normal bone marrow

·      more specific than gallium for infection

interpretation
Normal increased uptake

Child and adolescent

·      growth plates

·      more globular in younger children

Adult

·      thoracic and lumbar spine (greater than in cervical spine or sacrum)

·      anterior superior iliac spines

·      anterior wing of ilium

·      sacroiliac joints

·      shoulder joints (esp. dominant shoulder)

·      inferior wings of scapulae

·      sternoclavicular area

·      calcified tracheal, laryngeal and costal cartilages

Bladder

·      radionuclide excreted in urine

·      the fuller the bladder the larger the bladder image and the poorer the visualisation of the pelvis

·      patient may need to be catheterised if pelvic pathology suspected

Renal uptake

·      absent or very faint renal images may indicate superscan

·      abnormally high but symmetrical uptake in skeleton

·      may occur in metabolic bone disease or multiple diffuse skeletal metastases

·      may make incidental diagnosis

·      pelvic or ureteric obstruction

·      unilateral functional impairment of kidney

Artefacts

Injection site

·      commonest artefact

Urine

·      at external meatus, on thigh or on other site

·      may be problems with contaminated clothes in incontinent patient

Bladder diverticulum

·      may simulate metastatic involvement of anterior pelvis

Incomplete labelling

·      labelling may be incomplete so unbound pertechnate is available

·      free pertechnate accumulates in stomach, bowel, salivary glands  and thyroid gland

Superimposition of uptake

·      inferior angle of scapula may overlie rib on one side and not the other and simulate pathological region

Soft tissues

·      metastatic calcification

·      lungs

·      liver

·      infarction (cerebral, myocardial)

·      fat necrosis

·      myonecrosis, muscle haematoma or abscess

Normal decreased uptake

Artefacts

Metal objects

·      pendants, buckles, coins

·      external splints

·      implants for replacements and internal fixation

Equipment failure

·      abnormalities in crystal or photomultiplier tubes

Abnormal increased uptake

Malignant disease

·      skeletal metastases

·      primary malignant bone tumours

·      soft tissue metastases from osteosarcoma

Benign bone lesions

·      benign tumours associated with new bone formation

·      infection (acute or chronic)

·      arthridities

·      fractures and osteotomies

·      bone grafts

·      Paget's disease

·      miscellaneous

·      osteomalacia

·      hypertrophic pulmonary osteoarthropathy

Abnormal decreased uptake

Irradiation

Avascular lesions

·      Perthes disease

·      avascular necrosis

Occasional tumours

·      multiple myeloma

·      occasional metastases

Anatomical defects

·      amputations

·      congenital anomalies

Absence of activity

·      scleroderma

·      Buerger's disease

·      neuropathy

indications
Tumours

Skeletal metastases

·      most common indication

·      most active and sensitive method for detection

·      uses

·      diagnosis of symptomatic lesion

·      assessment of extent of dissemination of neoplasm

·      multiple myeloma often not detected

·      lesion does not provoke osteoblastic reaction

Primary bone tumours

·      use not as extensive

·      uses

·      extent of tumour

·      identification of skip lesions and spread

·      presence and location of some tumours like osteoid osteoma

·      identification of malignant change (eg diaphyseal aclasis)

Arthridities

Polyarthritis

·      most sensitive for early diagnosis and distribution of polyarthritis

·      nonspecific and thus not diagnostic alone

Ankylosing spondylitis

·      quantitative estimation of uptake in sacroiliac joints can assist with diagnosis

·      indication of response to treatment

Osteoarthritis

·      of little use

Infection

Acute osteomyelitis

·      useful in early diagnosis and localisation

·      may be negative in first 24 hours but usually positive at 3 days

·      increase in both blood pool and skeletal phases

·      in soft tissue infection, only blood pool phase increased

·      pitfalls in interpretation can occur with

·      fracture

·      septic arthritis

·      prior antibiotic treatment

·      unreliable in neonates

·      specificity can be increased by combination with Gallium or white cell labelled scan

·      white cells localised in infection

·      not reliant on reaction of bone to infection with increased osteoblastic activity

·      particularly useful for lesions near the growth plate

Chronic osteomyelitis

·      useful to assess whether new pain is due to acute flare-up or soft tissue infection

Septic arthritis

·      slightly less sensitive

·      useful to localise site of infection

·      esp. in children with hip pain

·      increased sensitivity when combined with Gallium or white cell labelled scan

·      does not replace need for joint aspiration

Joint prostheses

·      normal bone reaction around cemented components subsides after 9-12 months

·      persists longer in 20% of patients

·      bone reaction may be more prolonged in cementless prostheses

·      diagnostic scanning of little use under 12 months post-surgery

·      used to detect and differentiate infection and loosening

·      infection characterised by increased uptake in vascular phase but this is not pathognomic

·      Gallium and white cell labelled scan may be more specific for infection

Fractures

·      secondary to plain x-rays

·      useful in diagnosis of

·      recent vertebral crush fractures in osteoporotic patient with multiple compressed vertebrae

·      demented patient in pain with possibility of fracture somewhere

·      scaphoid tenderness with normal x-rays

·      femoral neck fractures in the elderly

·      stress fractures

·      usually becomes positive within 24 hrs

·      slower response in older and osteoporotic patients

·      preferable to wait 3-7 days

·      remains positive for variable period

·      depends on degree of repair and remodelling

·      may remain positive for several years

Avascular necrosis

Femoral head

·      in adult AVN and Perthes disease

·      initial diminished uptake

·      later patchy increased uptake

·      changes precede x-ray changes

·      bone scan 4 weeks after internal fixation of subcapital fracture is of prognostic significance

·      enhanced uptake associated with uncomplicated healing

·      nonunion and collapse associated with reduced uptake

·      not reliable earlier

Knee

·      useful in

·      osteochondritis dessicans

·      idiopathic osteonecrosis of femoral condyle

Other

·      Kienboch's disease of carpal lunate

·      Kohler's disease of tarsal navicular

Reflex sympathetic dystrophy

·      early findings are increase in both blood pool and delayed images

·      chronic RSD shows decrease in both blood pool and delayed images

Paget's disease

·      useful to establish extent of skeletal involvement and response to therapy

·      not useful in detecting fractures or sarcomatous change because of high uptake with or without these complication